Pseudomonas aeruginosa PAO1, PA5204 (argA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5204
Name
argA
Replicon chromosome
Genomic location 5858158 - 5859456 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253891.1
GI 15600397
Affymetrix PA5204_argA_at
Entrez 879475
GenBank AAG08589.1
INSDC AAG08589.1
NCBI Locus Tag PA5204
protein_id(GenBank) gb|AAG08589.1|AE004933_5|gnl|PseudoCAP|PA5204
TIGR NTL03PA05205
UniParc UPI0000125E00
UniProtKB Acc P22567
UniProtKB ID ARGA_PSEAE
UniRef100 UniRef100_B7V594
UniRef50 UniRef50_Q6D8H5
UniRef90 UniRef90_B7V594

Product

Feature Type CDS
Coding Frame 1
Product Name
N-acetylglutamate synthase
Product Name Confidence: Class 1
Synonyms amino-acid acetyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.00
Kyte-Doolittle Hydrophobicity Value -0.148
Molecular Weight (kDa) 47.9
Isoelectric Point (pI) 6.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 477 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004788 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5204
Search term: argA

Human Homologs

References

Complete nucleotide sequence of the Escherichia coli argA gene.
Brown K, Finch PW, Hickson ID, Emmerson PT
Nucleic Acids Res 1987 Dec 23;15(24):10586
PubMed ID: 3320971
Use of inducible feedback-resistant N-acetylglutamate synthetase (argA) genes for enhanced arginine biosynthesis by genetically engineered Escherichia coli K-12 strains.
Rajagopal BS, DePonte J 3rd, Tuchman M, Malamy MH
Appl Environ Microbiol 1998 May;64(5):1805-11
PubMed ID: 9572954
Functional dissection of N-acetylglutamate synthase (ArgA) of Pseudomonas aeruginosa and restoration of its ancestral N-acetylglutamate kinase activity.
Sancho-Vaello E, Fernández-Murga ML, Rubio V
J Bacteriol 2012 Jun;194(11):2791-801
PubMed ID: 22447897