Pseudomonas aeruginosa PAO1, PA5317 (dppA5)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5317
Name
dppA5
Replicon chromosome
Genomic location 5986475 - 5988055 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq NP_254004.1
GI 15600510
Affymetrix PA5317_at
Entrez 880892
GenBank AAG08702.1
INSDC AAG08702.1
NCBI Locus Tag PA5317
protein_id(GenBank) gb|AAG08702.1|AE004944_6|gnl|PseudoCAP|PA5317
TIGR NTL03PA05318
UniParc UPI00000C5FA2
UniProtKB Acc Q9HTN7
UniProtKB ID Q9HTN7_PSEAE
UniRef100 UniRef100_S0HDK8
UniRef50 UniRef50_W6R3H6
UniRef90 UniRef90_A6VEC4

Product

Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC dipeptide transporter
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.22
Kyte-Doolittle Hydrophobicity Value -0.226
Molecular Weight (kDa) 57.9
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 548 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004888 (532 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5317
Search term: dppA5

Transposon Insertions in PAO1

Type ISphoA/hah insertion site
Mutant ID UWGC: 32922
Genomic Position 5987377
Position in ORF 903(1581)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 8853
Genomic Position 5987828
Position in ORF 1354(1581)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_52_E12
Genomic Position 5987659
Position in ORF 1185(1581)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 30472
Genomic Position 5986822
Position in ORF 348(1581)
Reference 14617778

Transposon Insertions in Orthologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Peptide transport and chemotaxis in Escherichia coli and Salmonella typhimurium: characterization of the dipeptide permease (Dpp) and the dipeptide-binding protein.
Abouhamad WN, Manson M, Gibson MM, Higgins CF
Mol. Microbiol. 1991 May;5(5):1035-47
PubMed ID: 1956284
Crystal structure of the dipeptide binding protein from Escherichia coli involved in active transport and chemotaxis.
Dunten P, Mowbray SL
Protein Sci. 1995 Nov;4(11):2327-34
PubMed ID: 8563629
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D, Lafon C, Braun Y, Köhler T, Page MG, Mourez M, Weingart H
PLoS ONE 2014 ;9(10):e111311
PubMed ID: 25338022