Pseudomonas aeruginosa PAO1, PA2657 (bqsR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2657
Name
bqsR
Synonym: carR
Replicon chromosome
Genomic location 3007339 - 3008010 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
CommentFirst described as BqsR in PMID:19513205 (2008). Later referred to as CarR in PMID:26755627 (2016). According to pseudomonas.com guidelines, earlier gene name takes precedence if it appeared in a peer-reviewed scientific publication.

Cross-References

RefSeq NP_251347.1
GI 15597853
Affymetrix PA2657_at
DNASU PaCD00007145
Entrez 882366
GenBank AAG06045.1
INSDC AAG06045.1
NCBI Locus Tag PA2657
protein_id(GenBank) gb|AAG06045.1|AE004694_8|gnl|PseudoCAP|PA2657
TIGR NTL03PA02657
UniParc UPI00000C5791
UniProtKB Acc Q9I0I1
UniProtKB ID Q9I0I1_PSEAE
UniRef100 UniRef100_A0A072ZP29
UniRef50 UniRef50_Q0C2L9
UniRef90 UniRef90_A6V4C2

Product

Feature Type CDS
Coding Frame 1
Product Name
two-component response regulator BqsR
Product Name Confidence: Class 1
Synonyms two-component response regulator CarR
probable two-component response regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.14
Kyte-Doolittle Hydrophobicity Value -0.200
Molecular Weight (kDa) 24.7
Isoelectric Point (pI) 5.85

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 623 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002900 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2657
Search term: bqsR

Human Homologs

References

The Pseudomonas aeruginosa PAO1 Two-Component Regulator CarSR Regulates Calcium Homeostasis and Calcium-Induced Virulence Factor Production through Its Regulatory Targets CarO and CarP.
Guragain M, King MM, Williamson KS, Pérez-Osorio AC, Akiyama T, Khanam S, Patrauchan MA, Franklin MJ
J Bacteriol 2016 Jan 11;198(6):951-63
PubMed ID: 26755627
A novel two-component system BqsS-BqsR modulates quorum sensing-dependent biofilm decay in Pseudomonas aeruginosa.
Dong YH, Zhang XF, An SW, Xu JL, Zhang LH
Commun Integr Biol 2008;1(1):88-96
PubMed ID: 19513205
The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance.
Kreamer NN, Costa F, Newman DK
mBio 2015 Feb 24;6(2):e02549
PubMed ID: 25714721
BqsR/BqsS constitute a two-component system that senses extracellular Fe(II) in Pseudomonas aeruginosa.
Kreamer NN, Wilks JC, Marlow JJ, Coleman ML, Newman DK
J Bacteriol 2012 Mar;194(5):1195-204
PubMed ID: 22194456