Pseudomonas aeruginosa PAO1, PA5388 (caiX)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5388
Name	caiX
Replicon	chromosome
Genomic location	6065856 - 6066794 (- strand)
Transposon Mutants	3 transposon mutants in PAO1
Comment	Deletion of PA5388 or transposon insertion into the coding sequence results in a strain unable to grow using L-carnitine as a sole carbon source.
Comment	This gene has been experimentally demonstrated to be the carnitine transport periplasmic binding protein in P. aeruginosa.
Comment	Binds carnitine

Cross-References

RefSeq	NP_254075.1
GI	15600581
Affymetrix	PA5388_at
Entrez	880509
GenBank	AAG08773.1
INSDC	AAG08773.1
NCBI Locus Tag	PA5388
protein_id(GenBank)	gb|AAG08773.1|AE004951_7|gnl|PseudoCAP|PA5388
TIGR	NTL03PA05389
UniParc	UPI00000C5FD9
UniProtKB Acc	Q9HTH6
UniProtKB ID	Q9HTH6_PSEAE
UniRef100	UniRef100_W0Z3J4
UniRef50	UniRef50_G8Q1M3
UniRef90	UniRef90_A6VEK0

Product

Feature Type	CDS
Coding Frame	1
Product Name	CaiX Product Name Confidence: Class 1
Synonyms	carnitine-specific binding protein
Evidence for Translation
Charge (pH 7)	3.38
Kyte-Doolittle Hydrophobicity Value	-0.081
Molecular Weight (kDa)	33.8
Isoelectric Point (pI)	8.75

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 145 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004947 (484 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5388 Search term: caiX Search term: CaiX

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds. Chen C, Malek AA, Wargo MJ, Hogan DA, Beattie GA Mol Microbiol 2010 Jan;75(1):29-45 PubMed ID: 19919675