Pseudomonas aeruginosa PAO1, PA2657 (bqsR)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA2657
Name	bqsR Synonym: carR
Replicon	chromosome
Genomic location	3007339 - 3008010 (- strand)
Transposon Mutants	5 transposon mutants in PAO1
Comment	First described as BqsR in PMID:19513205 (2008). Later referred to as CarR in PMID:26755627 (2016). According to pseudomonas.com guidelines, earlier gene name takes precedence if it appeared in a peer-reviewed scientific publication.

Cross-References

RefSeq	NP_251347.1
GI	15597853
Affymetrix	PA2657_at
DNASU	PaCD00007145
Entrez	882366
GenBank	AAG06045.1
INSDC	AAG06045.1
NCBI Locus Tag	PA2657
protein_id(GenBank)	gb|AAG06045.1|AE004694_8|gnl|PseudoCAP|PA2657
TIGR	NTL03PA02657
UniParc	UPI00000C5791
UniProtKB Acc	Q9I0I1
UniProtKB ID	Q9I0I1_PSEAE
UniRef100	UniRef100_A0A072ZP29
UniRef50	UniRef50_Q0C2L9
UniRef90	UniRef90_A6V4C2

Product

Feature Type	CDS
Coding Frame	1
Product Name	two-component response regulator BqsR Product Name Confidence: Class 1
Synonyms	two-component response regulator CarR probable two-component response regulator
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-5.14
Kyte-Doolittle Hydrophobicity Value	-0.200
Molecular Weight (kDa)	24.7
Isoelectric Point (pI)	5.85

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543 Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 623 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002900 (533 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA2657 Search term: bqsR Search term: two-component response regulator BqsR

Human Homologs

References

The Pseudomonas aeruginosa PAO1 Two-Component Regulator CarSR Regulates Calcium Homeostasis and Calcium-Induced Virulence Factor Production through Its Regulatory Targets CarO and CarP. Guragain M, King MM, Williamson KS, Pérez-Osorio AC, Akiyama T, Khanam S, Patrauchan MA, Franklin MJ J Bacteriol 2016 Jan 11;198(6):951-63 PubMed ID: 26755627

A novel two-component system BqsS-BqsR modulates quorum sensing-dependent biofilm decay in Pseudomonas aeruginosa. Dong YH, Zhang XF, An SW, Xu JL, Zhang LH Commun Integr Biol 2008;1(1):88-96 PubMed ID: 19513205

The ferrous iron-responsive BqsRS two-component system activates genes that promote cationic stress tolerance. Kreamer NN, Costa F, Newman DK mBio 2015 Feb 24;6(2):e02549 PubMed ID: 25714721

BqsR/BqsS constitute a two-component system that senses extracellular Fe(II) in Pseudomonas aeruginosa. Kreamer NN, Wilks JC, Marlow JJ, Coleman ML, Newman DK J Bacteriol 2012 Mar;194(5):1195-204 PubMed ID: 22194456