Pseudomonas aeruginosa PAO1, PA3866

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3866
Name
Replicon chromosome
Genomic location 4327697 - 4329991 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252555.1
GI 15599061
Affymetrix PA3866_at
Entrez 879794
GenBank AAG07253.1
INSDC AAG07253.1
NCBI Locus Tag PA3866
protein_id(GenBank) gb|AAG07253.1|AE004804_1|gnl|PseudoCAP|PA3866
TIGR NTL03PA03866
UniParc UPI00000C5B4E
UniProtKB Acc Q9HXE0
UniProtKB ID Q9HXE0_PSEAE
UniRef100 UniRef100_Q9HXE0
UniRef50 UniRef50_Q9HXE0
UniRef90 UniRef90_Q9HXE0

Product

Feature Type CDS
Coding Frame 1
Product Name
Pyocin S4
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.99
Kyte-Doolittle Hydrophobicity Value -0.273
Molecular Weight (kDa) 80.7
Isoelectric Point (pI) 8.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5ODW PROTEIN TRANSPORT 07/06/17 Structure of the FpvAI-pyocin S2 complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.8 X-RAY DIFFRACTION 92.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 15 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000948 (428 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3866
Search term: Pyocin S4

Human Homologs

References

Novel bacteriocins with predicted tRNase and pore-forming activities in Pseudomonas aeruginosa PAO1.
Parret A, De Mot R
Mol Microbiol 2000 Jan;35(2):472-3
PubMed ID: 10652108
Molecular characterization of pyocin S3, a novel S-type pyocin from Pseudomonas aeruginosa.
Duport C, Baysse C, Michel-Briand Y
J Biol Chem 1995 Apr 14;270(15):8920-7
PubMed ID: 7721800
The soluble pyocins S2 and S4 from Pseudomonas aeruginosa bind to the same FpvAI receptor.
Elfarash A, Wei Q, Cornelis P
Microbiologyopen 2012 Sep;1(3):268-75
PubMed ID: 23170226