Pseudomonas aeruginosa PAO1, PA4006 (nadD1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4006
Name
nadD1
Synonym: ybeN
Synonym: nadD
Replicon chromosome
Genomic location 4486203 - 4486847 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_252695.1
GI 15599201
Affymetrix PA4006_at
Entrez 878969
GenBank AAG07393.1
INSDC AAG07393.1
NCBI Locus Tag PA4006
protein_id(GenBank) gb|AAG07393.1|AE004817_17|gnl|PseudoCAP|PA4006
TIGR NTL03PA04006
UniParc UPI000012FCB1
UniProtKB Acc Q9HX21
UniProtKB ID NADD_PSEAE
UniRef100 UniRef100_B7V8A6
UniRef50 UniRef50_Q4K5G0
UniRef90 UniRef90_A6V0A4

Product

Feature Type CDS
Coding Frame 1
Product Name
nicotinate mononucleotide adenylyltransferase NadD1
Product Name Confidence: Class 2
Evidence for Translation
Charge (pH 7) -2.90
Kyte-Doolittle Hydrophobicity Value -0.147
Molecular Weight (kDa) 23.8
Isoelectric Point (pI) 6.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1YUM TRANSFERASE 02/14/05 Crystal Structure of Nicotinic Acid Mononucleotide Adenylyltransferase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 100.0
1YUL TRANSFERASE 02/14/05 Crystal Structure of Nicotinic Acid Mononucleotide Adenylyltransferase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1YUN TRANSFERASE 02/14/05 Crystal Structure of Nicotinic Acid Mononucleotide Adenylyltransferase from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 514 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001677 (572 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4006
Search term: nadD1

Transposon Insertions in PAO1

Type ISlacZ/hah insertion site
Mutant ID UWGC: 10336
Genomic Position 4486249
Position in ORF 47(645)
Reference 14617778
Type Confirmed ISlacZ/hah insertion site
Mutant ID UWGC: 5108
Genomic Position 4486656
Position in ORF 454(645)
Reference 14617778

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_12020
Mutant ID PA14NR: 52612
Genomic Position 1039406
Position in ORF 4(645)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_12020
Mutant ID PA14NR: 34338
Genomic Position 1039652
Position in ORF 250(645)
Reference 16477005

References

Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene.
Mehl RA, Kinsland C, Begley TP.
J Bacteriol. 2000 Aug;182(15):4372-4.
PubMed ID: 10894752
The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y.
Science. 1997 Sep 5;277(5331):1453-62.
PubMed ID: 9278503
Identification and characterization of YLR328W, the Saccharomyces cerevisiae structural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme.
Emanuelli M, Carnevali F, Lorenzi M, Raffaelli N, Amici A, Ruggieri S, Magni G.
FEBS Lett. 1999 Jul 16;455(1-2):13-7.
PubMed ID: 10428462
PubMed ID: 27865623