Pseudomonas aeruginosa PAO1, PA4497 (dppA2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA4497
Name
dppA2
Replicon chromosome
Genomic location 5033194 - 5034792 (+ strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253187.1
GI 15599693
Affymetrix PA4497_at
Entrez 881082
GenBank AAG07885.1
INSDC AAG07885.1
NCBI Locus Tag PA4497
protein_id(GenBank) gb|AAG07885.1|AE004863_5|gnl|PseudoCAP|PA4497
TIGR NTL03PA04498
UniParc UPI00000C5D39
UniProtKB Acc Q9HVS4
UniProtKB ID Q9HVS4_PSEAE
UniRef100 UniRef100_Q9HVS4
UniRef50 UniRef50_Q87WI5
UniRef90 UniRef90_V6ALA8

Product

Feature Type CDS
Coding Frame 1
Product Name
probable binding protein component of ABC transporter
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.02
Kyte-Doolittle Hydrophobicity Value -0.378
Molecular Weight (kDa) 59.5
Isoelectric Point (pI) 7.00

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 557 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004085 (311 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4497
Search term: dppA2

Transposon Insertions in PAO1

Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_83_C11
Genomic Position 5033553
Position in ORF 360(1599)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_80_H11
Genomic Position 5033553
Position in ORF 360(1599)
Reference 15805499
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_17_B9
Genomic Position 5033204
Position in ORF 11(1599)
Reference 15805499
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 30722
Genomic Position 5033820
Position in ORF 627(1599)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Transcriptional fusion)
Mutant ID UBC: PAO1_lux_16_B9
Genomic Position 5033204
Position in ORF 11(1599)
Reference 15805499
Type ISphoA/hah insertion site
Mutant ID UWGC: 31870
Genomic Position 5034120
Position in ORF 927(1599)
Reference 14617778
Type mini-Tn5 lux transposon insertion site (Non-transcriptional fusion)
Mutant ID UBC: PAO1_lux_38_A11
Genomic Position 5033553
Position in ORF 360(1599)
Reference 15805499

Transposon Insertions in Orthologs

Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_58360
Mutant ID PA14NR: 42056
Genomic Position 5196813
Position in ORF 38(1599)
Reference 16477005
Type MAR2xT7 transposon (wild type background) insertion site
Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag PA14_58360
Mutant ID PA14NR: 39189
Genomic Position 5196950
Position in ORF 175(1599)
Reference 16477005

References

High-throughput screening of dipeptide utilization mediated by the ABC transporter DppBCDF and its substrate-binding proteins DppA1-A5 in Pseudomonas aeruginosa.
Pletzer D(1), Lafon C(2), Braun Y(1), Köhler T(3), Page MG(4), Mourez M(2), Weingart H(1).
PLoS One. 2014 Oct 22;9(10):e111311.
PubMed ID: 25338022