Pseudomonas aeruginosa PAO1, PA5147 (mutY)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5147
Name
mutY
Synonym: mutM
Synonym: micA
Replicon chromosome
Genomic location 5795954 - 5797021 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_253834.1
GI 15600340
Affymetrix PA5147_mutY_at
Entrez 878753
GenBank AAG08532.1
INSDC AAG08532.1
NCBI Locus Tag PA5147
protein_id(GenBank) gb|AAG08532.1|AE004927_10|gnl|PseudoCAP|PA5147
TIGR NTL03PA05148
UniParc UPI00000C5F25
UniProtKB Acc Q9HU37
UniProtKB ID Q9HU37_PSEAE
UniRef100 UniRef100_Q9HU37
UniRef50 UniRef50_Q9HU37
UniRef90 UniRef90_Q9HU37

Product

Feature Type CDS
Coding Frame 1
Product Name
A / G specific adenine glycosylase
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.29
Kyte-Doolittle Hydrophobicity Value -0.213
Molecular Weight (kDa) 39.3
Isoelectric Point (pI) 8.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 621 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004741 (558 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5147
Search term: mutY

Human Homologs

Ensembl 86, assembly GRCh38.p7
mutY DNA glycosylase [Source:HGNC Symbol;Acc:HGNC:7527]
E-value: 8.0e-47
Percent Identity: 46.3

Transposon Insertions in PAO1

Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 42586
Genomic Position 5796769
Position in ORF 816(1068)
Reference 14617778
Type Confirmed ISphoA/hah insertion site
Mutant ID UWGC: 39575
Genomic Position 5796304
Position in ORF 351(1068)
Reference 14617778

Transposon Insertions in Orthologs

References

MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III.
Michaels ML, Pham L, Nghiem Y, Cruz C, Miller JH
Nucleic Acids Res. 1990 Jul;18(13):3841-5
PubMed ID: 2197596
Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY.
Tsai-Wu JJ, Radicella JP, Lu AL
J. Bacteriol. 1991 Mar;173(6):1902-10
PubMed ID: 2001994
Characterization of the GO system of Pseudomonas aeruginosa.
Oliver A, Sánchez JM, Blázquez J
FEMS Microbiol. Lett. 2002 Nov;217(1):31-5
PubMed ID: 12445642