Pseudomonas aeruginosa PAO1, PA3045 (rocA2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3045
Name
rocA2
Replicon chromosome
Genomic location 3408560 - 3409183 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentRocA2 can interact with two sensors, RocS1 (PA3946) and RocS2 (PA3044). Signalling by RocS1 and RocS2 through RocA2 and leads to repression of the mexAB-oprM gene cluster and increased sensitivity to antibiotics.
CommentBacterial two hybrid studies in Pseudomonas aeruginosa PAK have shown that RocS1 and RocS2 can both interact with RocA2. Furthermore, a microarray study of PAK and PAK?rocA2, both overexpressing rocS2, revealed that RocA2 represses the expression of the mexAB-oprM gene cluster. qRT-PCR studies confirmed that the same signalling can be achieved by overexpressing rocS1, and in both cases the repression of mexAB-oprM led to an increased sensitivity to antibiotics.

Cross-References

RefSeq NP_251735.1
GI 15598241
Affymetrix PA3045_at
DNASU PaCD00007342
Entrez 882868
GenBank AAG06433.1
INSDC AAG06433.1
NCBI Locus Tag PA3045
protein_id(GenBank) gb|AAG06433.1|AE004729_7|gnl|PseudoCAP|PA3045
TIGR NTL03PA03045
UniParc UPI00000C58E5
UniProtKB Acc Q9HZG3
UniProtKB ID Q9HZG3_PSEAE
UniRef100 UniRef100_W0YS37
UniRef50 UniRef50_Q4K750
UniRef90 UniRef90_W0YS37

Product

Feature Type CDS
Coding Frame 1
Product Name
Two-component response regulator, RocA2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.56
Kyte-Doolittle Hydrophobicity Value 0.274
Molecular Weight (kDa) 22.5
Isoelectric Point (pI) 6.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 616 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000034 (777 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3045
Search term: rocA2

Human Homologs

References

Two-component regulatory systems in Pseudomonas aeruginosa: an intricate network mediating fimbrial and efflux pump gene expression.
Sivaneson M, Mikkelsen H, Ventre I, Bordi C, Filloux A
Mol Microbiol 2011 Mar;79(5):1353-66
PubMed ID: 21205015