Pseudomonas aeruginosa PAO1, PA3045 (rocA2)

Annotation Updates

Date	Description	Acknowledgements
Apr 29, 2002	Subcellular localization information added based on significance similarity to protein of known localization. Localization: Cytoplasmic. Localization Rating: Class 2.	Jennifer L. Gardy & Fiona Brinkman, Simon Fraser University, Canada
Aug 25, 2011	New product name: Two-component response regulator, RocA2. New gene name: rocA2. New product name confidence: Class 1. New comment : RocA2 can interact with two sensors, RocS1 (PA3946) and RocS2 (PA3044). Signalling by RocS1 and RocS2 through RocA2 and leads to repression of the mexAB-oprM gene cluster and increased sensitivity to antibiotics.. New comment : Bacterial two hybrid studies in Pseudomonas aeruginosa PAK have shown that RocS1 and RocS2 can both interact with RocA2. Furthermore, a microarray study of PAK and PAK?rocA2, both overexpressing rocS2, revealed that RocA2 represses the expression of the mexAB-oprM gene cluster. qRT-PCR studies confirmed that the same signalling can be achieved by overexpressing rocS1, and in both cases the repression of mexAB-oprM led to an increased sensitivity to antibiotics.. New PubMed reference: 21205015.	Allain Filloux, Imperial College London